14th Transgenic Technology Meeting

October 1-4, 2017 Salt Lake City,
Utah, USA Snowbird Resort

Program Schedule

View TT2017 program booklet View PDF version of program  Poster Corrections  

TT2017 Registration and Sessions will be held in the Cliff Lodge.

Sunday, October 1st, 2017

Council Meeting

10:00am – 2:00pm

Oktoberfest - Free Time

11:00am – 6:00pm

Registration Open - Cliff Lodge

2:00pm – 4:00pm

Welcome Address & Meeting Start

4:00pm – 4:30pm
Susan Tamowski and Jan Parker-Thornburg

Keynote Speaker: Alex Joyner, Sloan Kettering Institute, New York, USA

“Dissecting cerebellum development, regeneration and disease with mouse transgenic approaches”
4:30pm – 5:30pm

Introduced by: Jan Parker-Thornburg

Opening Reception at the Summit

6:00pm – 9:00pm

Monday, October 2nd, 2017

Continental Breakfast

8:00am – 8:30am

Keynote Speaker: Jin-Soo Kim, Seoul National University, Seoul, South Korea

“CRISPR genome editing in animals and plants”
8:30am -9:30am

Introduced by: Channabasavaiah Gurumurthy

Session 1: CRISPR optimization & traditional gene targeting

9:30am – 10:20am
Sponsored by: charles_river_logo
Chairs: Cheryl Bock & Aimee Stablewski

  1. Wojtek Auerbach, Regeneron, Tarrytown, NY, USA – In the Era of CRISPS are ES cells obsolete?”
  2. Simon Titen, University of Utah, Salt Lake City, USA – “Innovative strategies of traditional gene targeting”

Coffee Break

10:20am – 10:50am

Session 2: Novel CRIPSR genome engineering tools and technologies

10:50am – 12:05pm
Chair: Channabasavaiah Gurumurthy

  1. Channabasavaiah Gurumurthy, University of Nebraska, Omaha, NB, USA – “Latest trends in CRISPR mouse genome editing technologies”
  2. Tomoji Mashimo, Osaka University, Osaka, Japan – “CRISPR-mediated genome engineering in zygotes”
  3. Hiroshi Arakawa, Institute of Molecular Oncology, Milano, Italy – “A method to convert mRNA into a gRNA library for CRISPR/Cas9 editing of any organism”

Lunch and poster session (even numbers)

12:05pm – 1:30pm

Session 3: Panel: How to Run a Transgenic Core/Emerging CRISPR results

1:30pm – 3:30pm
Chair: Elizabeth Williams

  1. Ernst-Martin Fuechtbauer, Aarhus University, Aarhus, Denmark – Danish Centre for Transgenic Mice
  2. Willie Mark, Sloan Kettering Institute, New York, USA – Head of MSKCC Core Facility
  3. Branko Zevnik, University of Cologne, Cologne, Germany – Head of CECAD in vivo Research Facility
  4. Kathy Krentz, University of Wisconsin, Madison, WI, USA – Manager of Genome Editing and Animal Models core

Coffee Break

3:30pm – 4:00pm

Session 4: Non-mammalian transgenesis

4:00pm – 5:15pm
Chairs: Kristen Kwan & David Grunwald

  1. Kate O’Conner-Giles, University of Wisconsin, Madison, WI, USA – “CRISPR approaches for investigating novel neuronal gene in Drosophila”
  2. Lissa Herron, Roslin Institute, Edinburgh, Scotland – “The transgenic chicken bioreactor for valuable protein production”
  3. Matt Schwartz, University of Utah, Salt Lake City, USA – “SapTrap: high-throughput gene modification in C. elegans”

Special Session: ISTT Prize Introduction and Lecture

5:15pm – 6:15pm

Sponsored by:   genoway_logo

Introduced by: Jan Parker-Thornburg, Susan Tamowski and Dr. Yacine Cherifi, COO, genOway

Mario Capecchi, University of Utah, Salt Lake City, USA – “Gene Targeting into the 21st Century”

Vendor Reception and Poster Session #2 (odd numbers)

6:30pm – 8:30pm

Tuesday, October 3rd, 2017

Continental Breakfast

8:00am – 8:25am

Session 5: Large animal transgenesis; non-human primates

8:25am – 10:30am
Chair: Martina Crispo

  1. Irina Polejaeva, Utah State University, Logan, UT, USA – “Generation of Goat and Sheep Models for Biomedical and Agricultural Applications”
  2. Ana Paula Mulet/Alejo Menchaca, Institut Pasteur/IRAUy, Montevideo, Uruguay – “CRISPR/Cas9 system for genome editing in sheep”
  3. Tad Sonstegard, Recombinetics, Centerville, MD, USA – “Editing Livestock Genomes for Tropical Production”
  4. Sen Wu, China Agricultural University, Beijing, China – Generation of Pig Models for Biomedical Research
  5. Marina Emborg, University of Wisconsin, Madison, WI, USA – “Towards a genomic edited monkey model of Parkinson’s disease”

Coffee Break

10:30am – 11:00am

Session 6: Ethics & 3R’s

11:00am – 12:30pm
Chairs: Benoît Kanzler & Nikki Osborne
3R prize introduced by Boris Jerchow

  1. Nicole Duffee, AALAS, Memphis, TN, USA – How Did We Get Here?”
  2. Nikki Osborne, Responsible Research in Practice, West Sussex, England – “Opportunity Knocks”
  3. Boris Jerchow, University Medical Center Hamburg-Eppendorf (UKE), Germany – “Work to Be Done vs. Animal Welfare”
  4. 3R’s award winner: Roger Askew, Ozgene Pty Ltd., Lincoln, MA, USA – “Maximizing certainty of ES cell germline transmission rates – A cascade of efficiencies using genetically sterile “goGermline” embryos”
  5. Lin Wu, Harvard University, Cambridge, MA, USA – “CRISPR/Cas9 Gene Editing in Mice” *Selected speaker from abstracts

Lunch and Poster Viewing

12:30pm – 1:30pm

Session 7 - Selected speakers from abstracts

1:30pm – 3:00pm
Sponsored by: Print
Chair: Wojtek Auerbach

  1. Gary Kucera, Duke University, Durham, NC, USA – “Knocking Out Recombineering: An Innovative and Rapid Approach for the Targeted Generation of Complex Allelic Modifications”
  2. Kazhuto Yoshimi, Osaka University, Osaka, Japan – “Single strand DNA-mediated knock-in for large genomic regions in rodents”
  3. Denise Lanza, Baylor College of Medicine, Houston, TX, USA – “Employing single-stranded DNA oligonucleotides for the high throughput production of conditional knockout alleles in mice”
  4. Rachel Delston, Canopy Biosciences, St. Louis, MO, USA – “Targeted insertion of polyA tracks with CRISPR-Cas9 allows titratable control of gene expression”
  5. Katharina Boroviak – Wellcome Trust Sanger Institute, Cambridge, UK “Genomic rearrangements generated by CRISPR-Cas9 and their characterization pitfalls”
  6. Søren Warming, Genentech, San Francisco, CA, USA – “In-depth analysis of CRISPR off-targets in genetically engineered rodents”

Coffee Break

3:00pm – 3:30pm

Session 8: Advanced Genome Editing

3:30pm – 5:00pm
Chair: Lluís Montoliu

  1. Gaetan Burgio, Australian National University, Canberra, Australia – “Replications, ridicule and a recluse” NgAgo and beyond
  2. Maria Jasin, Sloan Kettering Institute, New York, USA – “Protecting the genome by homologous recombination”
  3. Tony Nolan, Imperial College London, London, England – “Using site-specific nucleases to build gene drives for suppression of populations of insect disease vectors”
  4. Lauryl Nutter, The Centre for Phenogenomics, Toronto, ON, Canada – “Cas9 RNA-guided nuclease – efficient model generation by microinjection and electroporation” *Selected speaker from abstracts

ISTT General Assembly

5:00pm – 6:00pm

TT2017 Banquet

7:00pm – 10:00pm

Wednesday, October 4th, 2017

Continental Breakfast

8:00am – 8:30am

Session 9: Using Animal Models to Understand Human Disease

8:30am – 10:10am
Chair: Lynn Doglio

  1. Zhongde Wang, Utah State University, Logan, UT, USA – “Genetically engineered golden Syrian hamsters as models of human disease”
  2. Monica Justice, University of Toronto, Toronto, Canada – “A mouse forward genetic modifier screen reveals therapies for Rett syndrome”
  3. Indira U. Mysorekar, Washington University, St. Louis, MO, USA – “Prevention of Zika Virus infections during pregnancy: Insights from mouse models”
  4. Randy Peterson, University of Utah, Salt Lake City, USA – Using zebrafish for in vivo drug discovery”

Coffee Break

10:10am – 10:40am

Session 10: Phenotyping/Imaging

10:40am – 12:10pm
Chairs: Peter Hohenstein & Branko Zevnik

  1. Nils Lindstrom, USC, Los Angeles, CA, USA – “Four-dimensional phenotypic analyses of mouse and human kidney development”
  2. Dario Valenzano, Max Planck Institute, Cologne, Germany – “The short-lived African turquoise killifish sheds light on the basis of vertebrate aging”
  3. Charlotte D’Hulst, Hunter College, NY, USA – “MouSensor: super sniffer mice to decode human olfaction”
  4. John Wiseman, AstraZeneca, Mölndal, Sweden – “Targeting human SerpinA1 by Cas9 therapeutic gene editing reverts liver damage phenotypes in a humanized α1-antitrypsin disease mouse model” *Selected speaker from abstracts

Lunch

12:10pm – 1:10pm

Keynote Speaker: Martin Cohn, University of Florida, USA - Orbis Pictus Lecture

“Development of external genitalia: from evolutionary origins to congenital malformations”
1:10pm – 2:00pm
Sponsored by: CCP logo

Introduced by: Inken Beck, Czech Centre for Phenogenomics & Charlie Murtaugh

Young Investigator Award

2:00pm – 2:30pm
Sponsored by:  Ingenious Targeting laboratory logo

Introduced by: Benoît Kanzler and Thomas Zeyda, inGenious Targeting Laboratory

Dr. Alexis Komor, Harvard University, Cambridge, MA, Young Investigator Awardee – “A New Approach to Genome Editing”

Poster Awards, Travel Awards, Final Remarks, Presentation of TT2019

2:30pm – 3:00pm

Poster Awards Sponsored by:  charles_river_logo

Travel Awards Sponsored by:   genentech250 logo

Presenters: Elizabeth Williams, Wojtek Auerbach, Xin Rairdan, Susan Tamowski, Yasuhide Furuta