14th Transgenic Technology Meeting

October 1-4, 2017 Salt Lake City,
Utah, USA Snowbird Resort

Workshops

Workshop #1 is full, but we are extending the registration application deadline for Workshop #2, the zebrafish workshop, to August 15, 2017.  Registration for the ISTT conference is not mandatory to participate in this workshop.  This is a hands-on workshop that will have some overlap with the mouse workshop running simultaneously regarding CRISPR design and synthesis of reagents.

Participation is subject to a selection process. To be eligible, you must upload a short CV, letter of motivation (max 200 words), and a short letter of support during conference registration.   Selected participants will be notified by email and charged the additional registration fee.

Note that Workshop 1 and Workshop 2 will have some combined lectures and sessions in addition to breakout hands-on sessions.

Suggested housing for the workshop is The University Guest House, the on-campus hotel at the University of Utah. A standard room will be $112.00/night plus tax. The workshops will be held in a nearby campus building, a short 10 minute walk from the hotel.

Workshop 1: Basics and latest trends in CRISPR mouse genome editing technologies                                

Sponsored by:charles_river_logo

The workshop will include lectures and demonstrations of CRISPR mouse genome engineering experiments using the advanced techniques such as Easi-CRISPR (Efficient additions with ssDNA inserts CRISPR) and GONAD (Genome editing via Oviductal Nucleic Acids Delivery). The participants will have opportunity to closely interact with the instructors and perform hands-on experiments such as designing CRISPR genome engineering projects, synthesis of CRISPR and donors reagents for microinjection and electroporation (GONAD) experiments, and either watch or perform microinjection and/or GONAD procedures.

Instructed by: Channabasavaiah Gurumurthy (Guru), Rolen Quadros, Masato Ohtsuka, Masahiro Sato & Donald Harms

Date: September 28, 1pm – October 1, 11am

Location: University of Utah

Participation Fee: $350

*Preliminary schedule:  to be updated

Thursday, September 28, 2017

1:00pmIntroduction, orientation (Susan Tamowski)
David Grunwald lecture, Recombination
Guru lecture, Advances in CRISPR genome editing technologies

Friday, September 29, 2017

8:30amOverview of Mouse genome engineering workshop (Guru)
9:00amLab session: synthesize donor DNA, RNA (Rolen, Guru)
12:00pmLUNCH
1:00pmDesign of gRNA/donors (Timothy)
2:00pmComputer lab session: design sgRNA to your favorite gene
4:00pmLecture/discussion (Guru)
Transgenesis and Tol2 vectors (Kristen)

Saturday, September 30, 2017

8:30amLab session: purify RNA, synthesize cDNA, purify ssDNA
10:00amGONAD lecture or microinjection session
12:00pmLUNCH
1:00pmMicroinjections/GONAD demo

Sunday, October 1, 2017

9:00amLook at injected embryos
Closing comments

Partial Workshop 1 Sponsor:  BEXLogo300ppi_95799

Workshop 2: An introduction to genome modification methods in the zebrafish

– Organized by Timothy Dahlem and David Grunwald

  • Assisted by Rich Dorsky, Kristen Kwan, Maurine Hobbs, Grunwald Lab, Kwan Lab

Date: September 28, 1pm – October 1, 11am

Location: University of Utah

Participation Fee: $350

*Preliminary schedule:  to be updated

Thursday, September 28, 2017

1:00pmIntroduction, orientation (Susan Tamowski)
David Grunwald lecture, Recombination
Guru lecture, Advances in CRISPR genome editing technologies

Friday, September 29, 2017

8:30amOverview of Zebrafish genome engineering workshop (Timothy Dahlem)
9:00amLab session: collection and injection of zebrafish embryos
12:00pmLUNCH
1:00pmDesign of gRNA/donors (Timothy)
2:00pmComputer lab session: design sgRNA to your favorite gene
3:30pmObserve embryo development; set up matings
4:00pmLecture/discussion (Guru)
Transgenesis and Tol2 vectors (Kristen)

Saturday, September 30, 2017

8:30amLab session: Injection of embryos
12:00pmLUNCH
1:00pmComputer lab session: donor design of your favorite gene
3:30pmObserve embryos development; previous day’s injections
4:00pmLecture/discussion (Josh Bonkowsky, Rich Dorsky)

Sunday, October 1, 2017

9:00amLook at injected embryos
Closing comments