14th Transgenic Technology Meeting

October 1-4, 2017 Salt Lake City,
Utah, USA Snowbird Resort

Workshops

Participation is subject to a selection process. To be eligible, you must upload a short CV, letter of motivation (max 200 words), and a short letter of support during conference registration.  Deadline to apply for the workshops is May 31, 2017. Selected participants will be notified by email and charged the additional registration fee.

Note that Workshop 1 and Workshop 2 will have some combined lectures and sessions in addition to breakout hands-on sessions.

Suggested housing for the workshop is The University Guest House, the on-campus hotel at the University of Utah. A standard room will be $112.00/night plus tax. The workshops will be held in a nearby campus building, a short 10 minute walk from the hotel.

Workshop 1: Basics and latest trends in CRISPR mouse genome editing technologies                                

Sponsored by:charles_river_logo

– Organized by Channabasavaiah Gurumurthy (Guru)

  • Assisted by Rolen Quadros, Tamowski lab, Mansour lab

Date: September 28, 1pm – October 1, 11am

Location: University of Utah

Participation Fee: $350

*Preliminary schedule:  to be updated

Thursday, September 28, 2017

1:00pmIntroduction, orientation (Susan Tamowski)
David Grunwald lecture, Recombination
Guru lecture, Advances in CRISPR genome editing technologies

Friday, September 29, 2017

8:30amOverview of Mouse genome engineering workshop (Guru)
9:00amLab session: synthesize donor DNA, RNA (Rolen, Guru)
12:00pmLUNCH
1:00pmDesign of gRNA/donors (Timothy)
2:00pmComputer lab session: design sgRNA to your favorite gene
4:00pmLecture/discussion (Guru)
Transgenesis and Tol2 vectors (Kristen)

Saturday, September 30, 2017

8:30amLab session: purify RNA, synthesize cDNA, purify ssDNA
10:00amGONAD lecture or microinjection session
12:00pmLUNCH
1:00pmMicroinjections/GONAD demo

Sunday, October 1, 2017

9:00amLook at injected embryos
Closing comments

Workshop 2: An introduction to genome modification methods in the zebrafish

– Organized by Timothy Dahlem and David Grunwald

  • Assisted by Rich Dorsky, Kristen Kwan, Maureen Hobbs, Grunwald Lab, Kwan Lab

Date: September 28, 1pm – October 1, 11am

Location: University of Utah

Participation Fee: $350

*Preliminary schedule:  to be updated

Thursday, September 28, 2017

1:00pmIntroduction, orientation (Susan Tamowski)
David Grunwald lecture, Recombination
Guru lecture, Advances in CRISPR genome editing technologies

Friday, September 29, 2017

8:30amOverview of Zebrafish genome engineering workshop (Timothy Dahlem)
9:00amLab session: collection and injection of zebrafish embryos
12:00pmLUNCH
1:00pmDesign of gRNA/donors (Timothy)
2:00pmComputer lab session: design sgRNA to your favorite gene
3:30pmObserve embryo development; set up matings
4:00pmLecture/discussion (Guru)
Transgenesis and Tol2 vectors (Kristen)

Saturday, September 30, 2017

8:30amLab session: Injection of embryos
12:00pmLUNCH
1:00pmComputer lab session: donor design of your favorite gene
3:30pmObserve embryos development; previous day’s injections
4:00pmLecture/discussion (Josh Bonkowsky, Rich Dorsky)

Sunday, October 1, 2017

9:00amLook at injected embryos
Closing comments